Practical laboratory guides and calculators.
100 mL recipes for common laboratory stock solutions, the working concentrations they are diluted to, converting between % w/v and molar, and a serial dilution calculator.
pKa values, useful pH ranges and temperature coefficients for 14 common laboratory buffers, how to choose between them, and a buffer finder for any target pH and working temperature.
Standard 1× and 10× PBS and 10 mM sodium citrate buffer recipes with a batch-size scaler, plus an acetate buffer calculator for pH 3.8 to 5.8.
Free dilution calculator (C1V1 = C2V2) and molarity calculator: work out stock volumes, final concentrations and how much solid to weigh out.
How to choose a syringe filter: 0.22 µm vs 0.45 µm pore size, and PES, nylon, PTFE, PVDF or cellulose acetate membranes, with good filtration practice.
How to aliquot buffers and reagent solutions into sterile vials: planning aliquot size, aseptic transfer, labelling and storage.
