1x Sodium Citrate Buffer, pH 6.0, 500mL Bottle

This Citrate Solution (antigen retriever) is a high‑purity, research‑grade buffer designed for heat‑induced epitope retrieval (HIER) in immunohistochemistry workflows. The solution is sterilized by 0.22 µm filtration and is DNase‑/RNase‑free, protease‑free, and pyrogen‑free, ensuring maximum reliability for sensitive tissue‑based staining procedures.

It is suitable for paraffin‑embedded sections and frozen tissue sections fixed using formaldehyde, paraformaldehyde, or other aldehyde‑based fixatives.

Key Features

• Type: Citrate Antigen Retrieval Buffer
• Sterility: 0.22 µm sterile‑filtered
• Nuclease‑free: DNase‑ and RNase‑free
• Protease‑free
• Pyrogen‑free
• Ready to use—no dilution required
• Compatible with aldehyde‑fixed tissues

Performance Characteristics

• Effectively breaks cross‑links formed by aldehyde fixatives
• Enhances epitope exposure in paraffin‑embedded tissue sections
• Significantly improves antibody binding and immunostaining clarity
• Consistent and reproducible results for IHC workflows
• Works with most standard HIER protocols (microwave, water bath, pressure cooker)

Applications

Suitable for:

• Paraffin‑embedded tissue section retrieval (primary application)
• Frozen tissue section retrieval
• Immunohistochemistry (IHC) workflows
• Antigen unmasking for various aldehyde‑fixed samples

Compatible fixatives include:

• Formaldehyde
• Paraformaldehyde
• Other aldehyde‑based reagents

Storage & Shelf Life

• Storage temperature: 2–30 °C
• Shelf life: 36 months (unopened)
• Handling: Keep tightly sealed; use aseptic technique after opening

Intended Use

For laboratory research use only.
Not for human or veterinary use.
Not for diagnostic, therapeutic, or medical applications.

Search‑Optimised Keywords

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