1x Sodium Citrate Buffer, pH 6.0, 500mL Bottle
Description:
This Citrate Solution (antigen retriever) is a high‑purity, research‑grade buffer designed for heat‑induced epitope retrieval (HIER) in immunohistochemistry workflows. The solution is sterilized by 0.22 µm filtration and is DNase‑/RNase‑free, protease‑free, and pyrogen‑free, ensuring maximum reliability for sensitive tissue‑based staining procedures.
It is suitable for paraffin‑embedded sections and frozen tissue sections fixed using formaldehyde, paraformaldehyde, or other aldehyde‑based fixatives.
Key Features
- Type: Citrate Antigen Retrieval Buffer
- Sterility: 0.22 µm sterile‑filtered
- Nuclease‑free: DNase‑ and RNase‑free
- Protease‑free
- Pyrogen‑free
- Ready to use—no dilution required
- Compatible with aldehyde‑fixed tissues
Performance Characteristics
- Effectively breaks cross‑links formed by aldehyde fixatives
- Enhances epitope exposure in paraffin‑embedded tissue sections
- Significantly improves antibody binding and immunostaining clarity
- Consistent and reproducible results for IHC workflows
- Works with most standard HIER protocols (microwave, water bath, pressure cooker)
Applications
Suitable for:
- Paraffin‑embedded tissue section retrieval (primary application)
- Frozen tissue section retrieval
- Immunohistochemistry (IHC) workflows
- Antigen unmasking for various aldehyde‑fixed samples
Compatible fixatives include:
- Formaldehyde
- Paraformaldehyde
- Other aldehyde‑based reagents
Storage & Shelf Life
- Storage temperature: 2–30 °C
- Shelf life: 36 months (unopened)
- Handling: Keep tightly sealed; use aseptic technique after opening
Intended Use
For laboratory research use only.
Not for human or veterinary use.
Not for diagnostic, therapeutic, or medical applications.
Search‑Optimised Keywords
Citrate antigen retrieval buffer, antigen retriever solution, HIER citrate buffer, paraffin section antigen retrieval, IHC buffer, DNase‑free buffer, RNase‑free citrate solution, aldehyde fixative antigen recovery, research‑grade antigen retrieval solution.
| Weight | 500 g |
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